Shimadzu ASMS 2014 Wednesday Breakfast Seminar 

“Pushing the Boundaries in Food and Microbiological Applications"

Dr. Philippa Hart

Applications Scientist, Shimadzu, Manchester, UK

Dr. Emmanuel Wey

Clinical Research Fellow, Royal Free NHS Trust, London, UK

Abstract: 

The use of MALDI-TOF-MS for standard bacterial typing at the species level has been extensively reviewed and is now widely accepted in the clinical environment.  Here we will aim to present a snapshot of how MALDI can be implemented beyond simple microbial identification.  This includes the in-depth characterization of microbial strains and their ability to confer resistance to a wide spectrum of -lactam antibiotics, but also the use of current library based approaches in food typing.

The presentation will begin by setting the scene from a clinical view point through the presentation of a typical clinical case.  We will then present an example of the way in which MALDI-TOF MS may be used to detect resistances markers. Finally, we will conclude by giving a brief example of how SARAMIS may be used to differentiate between types of milk and cheese, ultimately allowing us to detect evidence of food adulteration.