Contact
Registration Information
Carline Coote
FAES: 301-451-1434
registration@faes.org
Workshop Information
Mark Nardone
301-496-8290
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BioTech 56: RNA-Seq |
Biotechnology Training Courses at the National Institutes of Health (NIH)
RNA-seq or RNA sequencing is a new technology that utilizes the latest in next-generation sequencing approaches to obtain information about the presence/absence as well as the quantity of transcribed RNA (mRNA, rRNA, tRNA, or miRNA). Soon RNA-seq will be transplanting microarrays as the go-to procedure for analyzing the transcriptome of any genome. In this workshop, we will provide hands-on experience with RNA-seq - from the bench to the post-sequencing data acquisition (Illumina NextSeq) and analysis using the latest bioinformatics approaches. With a team of researchers from the NIH, area academic institutions and Illumina, we will cover examples of methodological approaches and applications of RNA-seq analysis to a variety of basic science and clinical biomedical research problems.
Lecture Topics
An Introduction to NGS and RNA-seq; Basics of RNA Sequencing and Analysis; Introduction to Downstream Analysis; RNA-seq Gene Expression Data Analysis Pipeline: Methods, Tools and Issues; Efficient Library Preparation from Embryonic Stem Cells (ESCs); Transcriptomic Changes in Human Brain Development; Bioconductor and RNA-seq Data Analysis; Integrating Gene Expression and Pathway Analysis in Developing Early Disease Biomarkers: A Genomic Approach; RNA-seq and CHIP-seq with Galaxy; Basic Downstream Analysis of RNA-seq and CHIP-seq Data with DAVID and IPA; Metaseq: A Python Package for Integrative Genome-wide Analysis; RNa seq Data Analysis in the Context of Biological Networks; RNA-seq of the Small RNAs of the Nucleolus; Transcriptome Profiling of CTLs using RNA-seq.
Laboratory Topics
Processing of RNA libraries; Load Sequencing Reactions; Recovery of Sequencing Data; Using BaseSpace to Analysis RNA-seq Data; Introduction to Linux, Sed, Awk and Bash Scripting; RNA-seq Analysis with the Tuxedo Package- Command Line
Other topics being considered but not confirmed: Circleator: Flexible Circular Visualization of Genome-associated Data with BioPerl and SVG; BioPerl and SVG
Related BioTech Courses
BioTech 40: Protein Bioinformatics
BioTech 45: Bioinformatic Analysis of Next Generation Sequencing Data
Registration Fees
$595.00 - NIH Students (fellows, post docs, post bacs)
$1,195.00 - NIH Employees/Government Employees/Academia
$1,795.00 - All Others
Comprehensive Binder
Contains all notes, protocols, and reference material needed. No additional books are required.
Academic Credit
Although no grades are given for BioTech Courses, each participant will receive Continuing Education Units (CEUs) based on the number of contact hours. Upon completion of this 5-day program, each participant will receive 3.5CEUs. Upon completion of each workshop, a certificate is issued.
Refund Policy
1. 100% tuition refund is available for registrations cancelled 14 or more days prior to the start of the workshop.
2. 50% tuition refund is available for registrations cancelled between 4 to 13 days prior to the start of the workshop. Instead of a partial refund, you may elect to take a full workshop credit, which can be applied to another BioTech program of similar length.
3. No refund is available for registrations cancelled 3 days or less prior to the start of the workshop. A full workshop credit will be available to you, which can be applied to another BioTech program of similar length.
All cancellations must be received in writing via fax to the FAES office, Attn. Ms.Carline Coote, 301-402-0174 during normal business hours (8:30 – 4:30 EST). Those received after hours are time marked for the following day. In the case of situation numbers 2 or 3, you must indicate whether you are opting for the full credit alternative at the time of written cancellation. In both cases, all payments will be processed by the start of the initial workshop.
Suggested Accommodations
The Double Tree Hotel & Executive Center