BioTech 49: Making iPSC From Blood
October 19 - 23, 2015

Biotechnology Training Courses at the National Institutes of Health (NIH)  

Recent advances in generating iPSCs now allow for their derivation from blood. This recent advance enables basic and clinical researchers to reprogram a blood cell into an iPSC and then further differentiate into any cell type. This capability allows researchers to develop "disease in a dish" paradigms to investigate disease and therapy mechanisms.

In this one week workshop, participants will learn how to generate iPSC from blood samples using a non-integrating approach. Due to the length of this procedure (iPSC generation ~3-4 weeks etc.) starting material (CD34+ cells or mononuclear cells) will be provided for each investigator and only critical stages will actually be performed during the laboratory portion of the workshop.

In addition to learning how to culture cells and reprogram blood cells into iPSCs, we will also present some of the latest methodologies for directing differentiation of these iPSCs into different lineages (e.g. hepatocytes or cardiomyocytes). Therefore, this course will package together the essential methodology to take a CD34+ cell isolated from blood, reprogram this cell, and then direct differentiation into multiple different lineages.

Lecture Topics
Introduction to Human Induced Pluripotent Stem Cells; Cellular Reprogramming Methodologies, Reprogramming from Blood; Specialized Media and Tools for Isolation and Enrichment of Blood-Derived Cells; Reprogramming using Cytotune Sendai Virus and Episomal Vectors; Differentiating IPSC to MSCs; Differentiating IPSC to RPEs; Overview of Validation Tests for Pluripotency.

Laboratory Topics
Observation of Monday’s cells and Cryopreservation; Maintenance of human iPSCs and Differentiation of iPSCs via Embryoid Bodies or Monolayer Platforms; iPS colony identification from TeSR-E7 Reprogramming Experiments; Making Embryoid Bodies Using Aggrewell; Neural Rosette Identification and Scoring; Reprogramming Using Cytotune Virus; Progression of Colony Formation via Fixed Dishes; Picking of Reprogrammed Colonies; Neon Electroporation, Transfection; Validation Tests for Pluripotency; RT-QPCR Assay for iPSC Characterization.

Related BioTech Courses
BioTech 18: Stem Cells
BioTech 47: Human Pluripotent Stem Cells (hiiPSC); Differentiation Neural Lineages
BioTech 54: Making Cardiomyocytes from iPSCs 
BioTech 55: Engineering with CRISPR, TALENs, and ZFNs
 

Training Rate
$1,795.00

Discounted Training Rates
$595.00 - NIH Trainees (fellows, postdocs, postbacs - NIH ONLY)
$1,295.00 - NIH Community
$1,595.00 - Academia, Government, Military

Comprehensive Binder
Contains all notes, protocols, and reference material needed. No additional books are required. 

Credit
Each participant will receive Continuing Education Units (CEUs) based on the number of contact hours. Upon completion of the course each participant will receive a certificate, showing completion of the workshop and 3.5 CEUs.  

Refund Policy
100% tuition refund or 100% full workshop credit is available for registrations cancelled 14 or more days prior to the start of the workshop.
50% tuition refund or 100% full workshop credit is available for registrations cancelled between 4 to 13 days prior to the start of the workshop. 
0% refund, 100% full workshop credit is available for registrations cancelled 3 days or less prior to the start of the workshop.  

Notification
1.  All cancellations must be received in writing via email to Ms. Carline Coote at ccoote@mail.nih.gov.
2.  Cancellations received after hours are time marked for the following day.  
3.  In regards to a full or partial refund or a full workshop credit, you must indicate which option you are selecting at the time of your written cancellation. 
4.  All refund payments will be processed by the start of the initial workshop.

Suggested Accommodations
The American Inn of Bethesda|
The Double Tree Hotel & Executive Center
Bethesda Hyatt Hotel 
Bethesda Court Hotel
Bethesda Marriott Hotel