BioTech 16: Expression, Detection & Purification of Recombinant Proteins in Prokaryotic and Eukaryotic Cells
October 14-16, 2015

 Biotechnology Training Courses at the National Institutes of Health (NIH

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This 3 day lecture laboratory course will give the student a good theoretical
background and practical experience in expression and purification of
recombinant proteins from a variety of expression systems. Furthermore, the
course will address new methods to overcome traditional challenges in
recombinant protein expression and purification. Finally, students will be
introduced to methods to prepare their samples for downstream applications such as immunofluorescence studies. 

Lecture and Laboratory Topics 
Introduction to Protein Purification Techniques; Expression of Proteins in
Prokaryotic and Eukaryotic Cells: Requirements, Advantages and Disadvantages; Prokaryotic Expression Systems: Strains, Tags and Purification Systems; Expression in Insect Cells: Baculovirus and Drosophila Systems; Expression in Mammalian Cells: Detection and Purification (Immunohistochemistry, Reporter Gene Assays, GFP, Tags for Expression/Purification Systems); Generation of Antibodies to Recombinant Proteins: System considerations and Antibody Purification; Expression in Yeast Cells (Pichia). System

Training Rate*
$ 1,295.00
*Multiple Registration Discount Available 

Discounted Training Rates
$ 395.00 - NIH Trainees (fellows, postdocs, postbacs - NIH ONLY)
$ 895.00 - NIH Community
$ 1,095.00 - Academia, Government, Military

Comprehensive Binder
Contains all notes, protocols, and reference material needed. No additional books are required. 

Credit
Each participant will receive Continuing Education Units (CEUs) based on the number of contact hours. Upon completion of this 3-day program, each participant will receive 2.1 CEUs. Upon completion of each workshop, a certificate is issued.

Refund Policy
1.  100% tuition refund is available for registrations cancelled 14 or more days prior to the start of the workshop. 
2.  50% tuition refund is available for registrations cancelled between 4 to 13 days prior to the start of the workshop. Instead of a partial refund, you may elect to take a full workshop credit, which can be applied to another BioTech program of similar length. 
3.  No refund is available for registrations cancelled 3 days or less prior to the start of the workshop.  A full workshop credit will be available to you, which can be applied to another BioTech program of similar length.
All cancellations must be received in writing Attn. Ms. Carline Coote; BioTech Registrar  during normal business hours (8:30 – 4:30 EST).  Those received after hours are time marked for the following day.  In the case of situation numbers 2 or 3, you must indicate whether you are opting for the full credit alternative at the time of written cancellation. In both cases, all payments will be processed by the start of the initial workshop.

Suggested Accommodations

The American Inn of Bethesda

The Double Tree Hotel & Executive Center

Bethesda Hyatt Hotel 

Bethesda Court Hotel

Bethesda Marriott Hotel

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