Where
The NIH Clinical Center,(Bldg.10) and
The John Porter Neuroscience Building (Bldg. 35A) Bethesda Campus
National Institutes of Health
Bethesda, MD 20892
Driving Directions
Contact
Boot Camp Information
Dr. Amy Hymes
Phone: 301-594-8985
Email: training@faes.org
Registration Information
Conference and Training Team
Phone: 301-451-1434
Email: registration@faes.org
Biophysical Methods 2015 Boot Camp
This workshop will review strategies for hydrodynamic and thermodynamic analysis of sedimentation equilibrium and sedimentation velocity analytical ultracentrifugation (AUC), dynamic light scattering (DLS), isothermal titration calorimetry (ITC), and fluorescence spectroscopy for the characterization of biological macromolecules in solution. The workshop will start on Monday morning and end on Friday at noon. On the first day, we will provide an introduction into the experimental aspects of AUC, DLS and ITC. This part will take place in the laboratory. We will review the basic operation of the instruments, choice of buffers, and detection systems, and experimental design dependent on the system under study. For AUC, assembling the AUC cells and operating the XLA/I will be discussed in detail. The following days will cover many advanced topics of theory, data analysis and experimental setup. These lectures are accompanied by practical computer exercises with the software. The workshop is aimed at researchers with at least limited experience in AUC, DLS and/or ITC. An integral part of the workshop is the presentation by the participants of their systems of interest and informal discussion of data.
Target Audience:
The workshop is aimed at researchers with at least limited experience in AUC, DLS, ITC, and/or fluorescence techniques for characterizing biomacromolecules.
Lecturers:
Andrea Balbo - Bioengineering and Physical Science Shared Resource, NIBIB, NIH
Chad Brautigam, Ph.D. - Department of Biophysics, UTSW Medical Center, Dallas, Texas
Rodolfo Ghirlando, Ph.D. - Laboratory of Molecular Biology, NIDDK, NIH
Grzegorz Piszczek, Ph.D. - Biophysics Core, NHLBI, NIH
Peter Schuck, Ph.D. - Dynamics of Macromolecular Assembly Section, LCIMB, NIBIB, NIH
Huaying Zhao, Ph.D. - Dynamics of Macromolecular Assembly Section, LCIMB, NIBIB, NIH
Registration Fees:
$1495.00 - Industry Participants (Pharmaceutical, Analytical and Commercial affiliate employees)
$895.00 - Academic Participants (P.I's, Professors, Staff Scientists, etc.)
$495.00 - Active Student Participants (Ph.D. Students, PostDocs, PostBacs, Fellows, IRTAs pending graduate or medical school) Documentation is Required.
Suggested Accommodations:
The Double Tree Hotel & Executive Center
Cancellations and Refund
Refunds will be issued for all cancellations, received in writing, on or before May 5th, 2015. Please contact Carline Coote at (301) 451-1434 or via email, registration@faes.org , if you wish to cancel your registration. No refunds will be issued after a May 5th, 2015.
Image courtesy of the National Institute of Health/Department of Health and Human Services. Use of image does not imply endorsement of any activity or product by the National Institutes of Health/Department of Health and Human Services.