BioTech 35: Immunofluorescence and          Confocal Microscopy
January 19-22, 2016

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Through morning classes and afternoon "hands-on" lab sessions, Biotech 35 will focus on two important methods that are used extensively in biomedical research.  Fluorescence microscopy is a useful tool for observing cellular morphology and function that is readily available and relatively simple to learn.  Confocal microscopy has emerged as a powerful and popular extension of fluorescence microscopy, allowing 3-dimensional localization and dynamics of cellular components.  The course is designed as a “boot camp” for those cell biologists just entering the world of confocal microscopy, wishing to utilize the technology to its fullest potential.  Several related, advanced topics are introduced to give participants an overview of future possibilities. 

Classroom Topics
Basic Theory and Practical Aspects of Fluorescence, Immunofluorescence, and Confocal Microscopy; Applications; Specimen Preparation; Spinning Disk Confocal; 2-Photon Excitation; Colocalization of Proteins: Using FRET to show protein-protein interactions; Spectral Unmixing; Live Cell Studies: Translocation and localization of proteins using fluorescent fusion proteins and time lapse imaging; Protein Dynamics Using FRAP (Fluorescence Recovery After Photobleaching), FLIP (Fluorescence Loss In Photobleaching), and PA-GFP (Photo-Activatible Green Fluorescent Protein); Image Processing and Analysis using Fiji. 

Brief classroom introductions without labs: SPIM (Selective Plane Illumination Microscopy, also called Light Sheet Microscopy); 3D Deconvolution; Super-resolution microscopy: SIM (Structured Ilumination Microscopy), STED (STimulated Emission Depletion), PALM (Photo-Activatable Light Microscopy), STORM (STochastical Optical Reconstruction Microscopy), GSD (Ground State Depletion), and PAINT (Point Accumulation for Imaging in Nanoscale Topography).

Laboratory Topics
Rotating in groups of 4 or less, participants will gain hands-on experience with: Sample preparation for Fluorescence/Confocal Microscopy; Acquisition/Optimization of Basic Confocal Scans using Single-point Laser-Scanning Confocal Microscopes, Multiphoton excitation, and Spinning Disk Confocal; Colocalization using multi-color fluorescence imaging; FRET; Spectral Unmixing; Live Cell Imaging: Translocation and localization of proteins using fluorescent fusion proteins and time lapse imaging; Protein Dynamics Using FRAP, FLIP, and PA-GFP; Image Processing and Analysis using Fiji;

Related Bio-Tech Course

BioTech 53: Super Resolution Microscopy- March 15-18, 2016 

 

TRAINING RATE:

$1,495.00
 

 DISCOUNTED RATES FOR:
$495.00 - NIH Trainees (fellows, postdocs, postbacs - NIH ONLY)
$1,095.00 - NIH Community
$1,295.00 - Academia, Government, Military 

Comprehensive Binder Included 
Contains all notes, protocols, and reference material needed. No additional books are required. 

Credit
Each participant will receive Continuing Education Units (CEUs) based on the number of contact hours. Upon completion of this 4-day program, each participant will receive 2.8 CEUs. Upon completion of each workshop, a certificate is issued.

Refund Policy

100% tuition refund or 100% full workshop credit is available for registrations cancelled 14 or more days prior to the start of the workshop. 

50% tuition refund or 100% full workshop credit is available for registrations cancelled between 4 to 13 days prior to the start of the workshop. 

0% refund, 100% full workshop credit is available for registrations cancelled 3 days or less prior to the start of the workshop.  

Suggested Accommodations

The American Inn of Bethesda

The Double Tree Hotel & Executive Center

Bethesda Hyatt Hotel 

Bethesda Court Hotel

Bethesda Marriott Hotel

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