BioTech 23: Flow Cytometry : Principles and Methods
February 23-26, 2016

Biotechnology Training Courses at the National Institutes of Health (NIH)  

 

Register Now!If you are an NIH employee paying with the signed vendor copy of the SF-182 form, please email:  registration@faes.org

 

This four day lecture/laboratory workshop will cover various applications of
flow cytometry in basic research. Selected lecture topics will provide a broad
background about the functions of a flow cytometer; choice of fluorochromes;
data analysis and presentation; technical protocols for flow cytometric
procedures and troubleshooting during data acquisition and analysis.

Lecture and Laboratory Topics
Basic Setup of Flow Cytometers; Principle of Cell Sorting; Parameters for  Sorting; Alternative Separation Techniques (Including Magnetic Bead Separation MACS); Choice of Fluorochromes Depending on the type of Instrument and Application; Parameters of Analysis, Data Analysis and Interpretation; In-depth Discussion of Examples of Various Flow Cytometric Assays (e.g. Cell Cycle Analysis, Cell Activation, Apoptosis, Immunology, Hematology, Biochemistry with Methodical Protocols); Multi-color Analysis (With Special Emphasis on How to Combine Flurochromes and Fluorescence Compensation); Choosing the Right Controls for Data Analysis (Isotype Controls; FMO controls; Blocking with Antibodies); Hands-on Laboratory with 4- and 8-color staining (BD Accuri and MACSQuant Analyzer); Data Workshop; Hands-on Data Analysis with samples From Cell Cycle Analysis ; Apoptosis; Multi-Color Surface Staining; Proliferation using Bio-Tracers; Software training: Introduction to FlowJo Cytometry Analysis Software.

General Training Rate
$1,495.00

Discounted Training Rates                                                                               $495.00 - NIH Trainees (fellows, postdocs, postbacs - NIH ONLY)                        $1,095.00 - NIH Community                                                                                    $1,295.00 - Academia, Government, Military

Comprehensive Binder
Contains all notes, protocols, and reference material needed. No additional books are required. 

Credit
Although no grades are given for BioTech Courses, each participant will receive Continuing Education Units (CEUs) based on the number of contact hours. Upon completion of this 4-day program, each participant will receive 2.8 CEUs. Upon completion of each workshop, a certificate is issued.

Refund Policy
100% Refund or 100% Full Workshop Credit Available                                     Written Notification: 14 or more days prior to start of program.

50% Refund or 100% Full Workshop Credit Available                                        Written Notificaiton: 4 to 13 Days prior to start of program.

 0% Refund, 100% Full Workshop Credit Available                                             3 Days or less prior to start of program.

 Notification

• All cancellations must be received in writing via email to Ms. Carline Coote at ccoote@mail.nih.gov
• Cancellations received after hours are time marked for the following day.
• In regards to a full or partial refund or a full workshop credit, you must indicate which option you are selecting at the time of your written cancellation.
• All refund payments will be processed by the start of the initial workshop.

Suggested Accommodations

The American Inn of Bethesda

The Double Tree Hotel & Executive Center

Bethesda Hyatt Hotel 

Bethesda Court Hotel

Bethesda Marriott Hotel

 

Return to BioTech Calendar