BioTech 47: iPSCII:  Human Induced Pluripotent Stem Cells (hiPSC): Differentiation to Neural Lineages 
                            March 7 - 11, 2016

           

          Biotechnology Training Courses at the National Institutes of Health (NIH)  

 

Register Now! 

If you are an NIH employee paying with the signed vendor copy of the SF-182 form, please email:  registration@faes.org

Induced pluripotent stem cells (iPSC) represent enormous potential in that they are capable of differentiating into virtually any cell type in the human body. Directing this differentiation into specific cell types in a consistent and efficient manner enables researchers to investigate new therapy and screening approaches in patient-derived cells. This 5 day hands-on training workshop will provide participants with the training and knowledge to help the researcher bring iPSC technology to the laboratory. Students will gain practical knowledge for developing new cell lines from different cell types. Lectures will discuss the expression of genes required for inducing pluripotency and methods of making (virus, RNA, plasmid) and maintaining iPS cells. Lectures on conditions needed for differentiating iPSC to neural, epithelial, and hematopoietic lineages will also be discussed. 

The emphasis of the course is on practical information that will help the investigator in the laboratory. Emphasis will be placed on deriving iPSC and differentiation to the neural lineage. Laboratory exercises are intense and will be directed by experts with a working knowledge of the techniques. Labs will cover methods for making iPSC and picking iPSC colonies. In situ analysis of pluripotency on live cells will also be conducted. Laboratory exercise on neural stem cells will include cryopreservation techniques and immunocytochemical analysis of pluripotent marker (Oct4) and neural markers (Sox1, Nestin and Sox2).

Topics 
Overview; Differentiation of Human Pluripotent Stem Cells into NSC; Differentiation Methods; Cell Culture Methods; Introduction to Mouse and Human Induced Pluripotent Stem Cells; Cellular Reprogramming Methodologies; mRNA Reprogramming of Patient Samples; Optimizing mRNA Reprogramming Efficiency; Differentiating IPSC to Retina Pigment Epithelium; Epigenetic Understanding of Pluripotency; Differentiating IPSC from CD34+ cells; Differentiating IPSC to the Hematopoietic Lineage

Differentiation of IPS to Neutrophils; Preparation of Cells From Embryoid Bodies To Study Differentiation; Differentiating IPSC to Neurons; Techniques Used for neural differentiation; Neural Induction and Neural Expansion; NSC Staining for Pluripotent Marker (Oct4) and Neural Markers (Sox1, Nestin and Sox2); Cryopreservation of Neural Stem Cells

Class size will be limited to 16 

General Training Rate
$1,795.00

Discounted Training Rates                                                                               $595.00 - NIH Trainees (fellows, postdocs, postbacs - NIH ONLY)                     $1,295.00 - NIH Community                                                                                      $1,595.00 - Academia, Government, Military

Comprehensive Binder
Contains all notes, protocols, and reference material needed. No additional books are required. 

Credit
Although no grades are given for courses, each participant will receive Continuing Education Units (CEUs) based on the number of contact hours. Upon completion of the course each participant will receive a certificate, showing completion of the workshop and 3.5 CEUs.    

Related BioTech Courses
BioTech 18: Stem Cells
BioTech CRM 49: Making iPSCs from Blood
BioTech CRM 54: Making Cardiomyocytes from iPSCs 
BioTech CRM 55: Engineering with CRISPR, TALENs, and ZFNs

Refund Policy                                                                                                              100% tuition refund or 100% full workshop credit is available for registrations cancelled 14 or more days prior to the start of the workshop. 

50% tuition refund or 100% full workshop credit is available for registrations cancelled between 4 to 13 days prior to the start of the workshop. 

0% refund, 100% full workshop credit is available for registrations cancelled 3 days or less prior to the start of the workshop.  

Notification

• All cancellations must be received in writing via email to Ms. Carline Coote at ccoote@mail.nih.gov.
• Cancellations received after hours are time marked for the following day.  
• In regards to a full or partial refund or a full workshop credit, you must indicate which option you are selecting at the time of your written cancellation. 
• All refund payments will be processed by the start of the initial workshop.

 

Suggested Accommodations

The American Inn of Bethesda

The Double Tree Hotel & Executive Center

Bethesda Hyatt Hotel 

Bethesda Court Hotel

Bethesda Marriott Hotel

 

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