BioTech 22: In Situ Hybridization           
April 19-22, 2016

Biotechnology Training Courses at the National Institutes of Health (NIH)  

This 4 day course is designed to introduce the participant to molecular hybridization and in situ hybridization techniques.  The application of these techniques to current research questions in genetics and gene expression, molecular pathology, and pathogen detection and identification will be discussed.  Probe application and detection systems will serve as the basis for both RNA and DNA in situ hybridization techniques to be addressed in lecture and laboratory.  This course will be staffed by clinical and basic scientists familiar with the applications of hybridization techniques to the problems of human disease.

Topics
Probe Design, Optimization, and Application at the Chemical and Cytological Levels; Principles of Hybridization; Base Structure, Modification, Stability and Specificity; Hybridization Kinetics; Preparation of Chemical and Cellular Samples; Hybridization Formats: Target Abundance vs. Probe Abundance, Spots/ Blots, Southern and Northern Analysis, Soluble Targets,in situ Hybridization; Radioactive, Biotinylated, DNP, Chemoluminescent and Oligonucleotide Probe Synthesis, Purification and Use; Label and Signal Amplification and Hybridization Accelerators.  DNA in situ hybridization (FISH); Interphase Molecular Cytogenetics; RNA in situ Hybridization; RT-PCR in situ Hybridization; Principles of Karyotyping and Metaphase Chromosome Preparation; Tissue and Specimen Preparation Fixatives, Comparative Genome Hybridization; Spectral Karyotyping; Fluorescence Microscopy; and in situ PCR.

Training Rate*
$1,495.00
*Multiple Registration Discount Available 

Discounted Training Rates
$495.00 - NIH Trainees (fellows, postdocs, postbacs - NIH ONLY)
$1,095.00 - NIH Community
$1,295.00 - Academia, Government, Military 

Comprehensive Included 
Contains all notes, protocols, and reference material needed. No additional books are required. 

Credit
Although no grades are given for courses, each participant will receive Continuing Education Units (CEUs) based on the number of contact hours. Upon completion of the course each participant will receive a certificate, showing completion of the workshop and CEU credits.  For a 3-day workshop, a participant receives 2.1 CEUs.  For a 4-day workshop, a participant receives 2.8 CEUs.  For a 5-day workshop, a participant receives 3.5 CEUs.  

Refund Policy
100% tuition refund or 100% full workshop credit is available for registrations cancelled 14 or more days prior to the start of the workshop. 
50% tuition refund or 100% full workshop credit is available for registrations cancelled between 4 to 13 days prior to the start of the workshop. 
0% refund, 100% full workshop credit is available for registrations cancelled 3 days or less prior to the start of the workshop.  

Notification
1.  All cancellations must be received in writing via email to Ms. Carline Coote at ccoote@mail.nih.gov.
2.  Cancellations received after hours are time marked for the following day.  
3.  In regards to a full or partial refund or a full workshop credit, you must indicate which option you are selecting at the time of your written cancellation. 
4.  All refund payments will be processed by the start of the initial workshop.

Suggested Accommodations
The American Inn of Bethesda
The Double Tree Hotel & Executive Center
Bethesda Hyatt Hotel 
Bethesda Court Hotel
Bethesda Marriott Hotel