BioTech 7: Animal and Human Cell Culture:  Method and Applications
May 9 - 13, 2016
                                         
Biotechnology Training Courses at the National Institutes of Health (NIH)  

 

Register Now!

If you are an NIH employee paying with the signed vendor copy of the SF-182 form, please email:  registration@faes.org

This lecture and laboratory course is structured to provide life scientists who are not experienced in cell culture with an introduction to principles and practices that will facilitate their ability to develop the use of in vitro systems. Additionally, investigators without formal training in tissue culture techniques will find the information and laboratory exercises extremely useful. This workshop is predicated on the application of the most rigorous principles of quality control and will be taught by experienced researchers with extensive years of experience in the field.

Lecture Topics
From Donor to Cell Lines: The in vitro environment: Setting up a Tissue Culture Facility, Equipment, Judicious use of Antibiotics, Mycoplasma Effects and Testing, Good Tissue Culture Practices, Cell Line Authentication and Cross Contamination, Nutrients and Plating Surfaces, Experimental Designs; Sources for Tissue Culture; Cell lines and Primary Culture, Adult, Embryonic, and Induced Pluripotent Stem Cell Lines, Tissue Culture from Different Species, Normal, Immortalized, Tumor Cell Lines, 3-dimensional Platforms; Cell Engineering, Transient and Permanent Transfections, Expression Constructs, Oligonucleotides, Viral Vectors, and Transductions; Properties of Normal and Transformed Cell, Finite Life Span, Low Density, Clonal Growth, Mutagenesis: Cell Repositories, ATCC, Rutgers, and Coriell Insitute for Medical Research; Toxicity Testing in the 21^st Century, Organs on a Chip, and Other Applications of in vitro Techniques. 

Laboratory Topics
Subculturing Different Cell Lines: Gross Cytology and Cell Counting, Staining; Cell Cycle and Mycoplasma Analysis; Cell Line Authentication; Karyotyping and Chromosome Breaks; Cloning: Cloning by Limit Dilution and Cloning Rings; Plating Efficiency and Colony Forming Assays; Transformed Cells: Growth Patterns,  Growth in Soft Agar and Collagen Gels; Cell Purification by Centrifugation and Cell Surface Antibody; Viability Assays, Fluorescent, Apoptotic, MTT, LDH and ATP Assays; Transfection and Transductions, Reporter Assays, Stem Cell Differentiation

Training Rate*
$1,795.00
*Multiple Registration Discount Available 

Discounted Training Rates
$595.00 - NIH Trainees (fellows, postdocs, postbacs - NIH ONLY)
$1,295.00 - NIH Community
$1,595.00 - Academia, Government, Military 

Comprehensive Binder
Contains all notes, protocols, and reference material needed. No additional books are required. 

Credit
Although no grades are given for courses, each participant will receive Continuing Education Units (CEUs) based on the number of contact hours. Upon completion of the course each participant will receive a certificate, showing completion of the workshop and CEU credits.  For a 3-day workshop, a participant receives 2.1 CEUs.  For a 4-day workshop, a participant receives 2.8 CEUs.  For a 5-day workshop, a participant receives 3.5 CEUs.  

Refund Policy
100% tuition refund or 100% full workshop credit is available for registrations cancelled 14 or more days prior to the start of the workshop. 
50% tuition refund or 100% full workshop credit is available for registrations cancelled between 4 to 13 days prior to the start of the workshop. 
0% refund, 100% full workshop credit is available for registrations cancelled 3 days or less prior to the start of the workshop.  

Notification
1.  All cancellations must be received in writing via email to Ms. Carline Coote at ccoote@mail.nih.gov.
2.  Cancellations received after hours are time marked for the following day.  
3.  In regards to a full or partial refund or a full workshop credit, you must indicate which option you are selecting at the time of your written cancellation. 
4.  All refund payments will be processed by the start of the initial workshop.

Suggested Accommodations
The American Inn of Bethesda
The Double Tree Hotel & Executive Center
Bethesda Hyatt Hotel 
Bethesda Court Hotel
Bethesda Marriott Hotel

 

 

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