BioTech 2: Recombinant DNA Methodology - Molecular Biology of Nucleic Acids: Fundamentals   & Applications                                                 June 7 - 10, 2016

Biotechnology Training Courses at the National Institutes of Health (NIH)  

 

If you are an NIH employee paying with the signed vendor copy of the SF-182 form, please email:  registration@faes.org

Register Now! 

This lecture and laboratory course is designed to provide the novice with an
introduction to the molecular biology of nucleic acids. An approach emphasizing both principles and methodology provides the scientist with the essential fundamentals needed for gene cloning and for an appreciation of the strategies of recombinant DNA technology as well as other molecular techniques.

Lecture Topics:
Introduction to Nucleic Acids and Molecular Cloning; Isolation and Purification of Nucleic Acids; Restriction Enzymes and Restriction Mapping; Other Pertinent Enzymes; Analysis of DNA and RNA Sequences; Northern and Southern Blot Analysis; Construction of Nucleic Acid Probes; Mapping; cDNA Synthesis Part I: cDNA Synthesis; Construction of cDNA Libraries; PCR-Derived cDNA Libraries; Enzymology; cDNA Synthesis Part II: Screening for Differentially Expressed Genes;  Microarrays;  Differential Display; Subtraction Hybridization; Subtraction Cloning; The Polymerase Chain Reaction Part I: Principles, Theory, Optimaization; Contamination; The Polymerase Chain Reaction (PCR), Part II – PCR Variations: In Situ PCR, Mutagenesis PCR, Real Time PCR, Digital PCR, PCR Arrays, PCR SSCP,  PCR Nested, and Muliplex PCR; DNA nad RNA Sequencing; Bioinformatics; Expression of Cloned Sequences and Their Assays; Epigenetics: Principles and Applications.  

Laboratory Topics:
Laboratory Session I:
Extraction of High Molecular Weight Genomic DNA from Mammalian Cells;
Restriction Enzyme Digestion of Recombinant Plasmid; Gel Electrophoresis:
Separation of Restriction Digestion Products on Agarose Gel Purification of
Restriction Fragment: Excise Fragment from Gel; Southern Analysis: Blot DNA to Membrane.

Laboratory Session II
Extraction of Total RNA from Mammalian Cells; RNA Gel
Electrophoresis; Isolation and Purification of DNA from Agarose Gel Slice;
Ligation of Purified DNA Fragment into Vector;

Laboratory Session III
Transformation of Bacteria; Southern Analysis: 3` End-Labeling
of Probe with Biotin; PCR: Amplification of Human SIX 6.

Laboratory Session IV
Extraction of Plasmid DNA from Bacteria; Agarose Gel
Electrophoresis of Plasmid and Chromosomal DNA; Southern Analysis:
Hybridization of Probe to Membrane and Chemiluminescent Detection; C. PCR: Electrophoresis and Analysis;

General Training Rate:                                                                                             $1,495.00 

Registration Fees
 $    495.00 - NIH Trainees (fellows, postdocs, postbacs - NIH ONLY)
 $1, 095.00 - NIH Community                                                                                            $1, 295.00 - Government Employees/Military/Academia                       

Comprehensive Binder Included
Contains all notes, protocols, and reference materials needed. No additional books are required. 

Credit
Although no grades are given for courses, each participant will receive Continuing Education Units (CEUs) based on the number of contact hours. Upon completion of the course each participant will receive a certificate, showing completion of the workshop and 2.8 CEUs.  

Refund Policy

100% tuition refund or 100% full workshop credit is available for registrations cancelled 14 or more days prior to the start of the workshop. 

50% tuition refund or 100% full workshop credit is available for registrations cancelled between 4 to 13 days prior to the start of the workshop. 

0% refund, 100% full workshop credit is available for registrations cancelled 3 days or less prior to the start of the workshop.  

Notification

• All cancellations must be received in writing via email to Ms. Carline Coote at ccoote@mail.nih.gov.
• Cancellations received after hours are time marked for the following day.  
• In regards to a full or partial refund or a full workshop credit, you must indicate which option you are selecting at the time of your written cancellation. 
• All refund payments will be processed by the start of the initial workshop.

 

Suggested Accommodations

The Double Tree Hotel & Executive Center

Bethesda Hyatt Hotel 

Bethesda Court Hotel

Bethesda Marriott Hotel

 

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