FAES@NIH
Where
National Institutes of Health 9000 Rockville Pike Bldg. 60, Room 036 Bethesda, MD 20892
Contact
Workshop Information
Conference and Training Team
Telephone: 301-496-7977
Email: registration@faes.org
BioTech 53: Super Resolution Microscopy: Principles and Methods
March 15-18, 2016
Biotechnology Training Courses at the National Institutes of Health (NIH), Bethesda, Maryland, USA
If you are an NIH employee paying with the signed vendor copy of the SF-182 form, please email: registration@faes.org
Register Now!
The course is designed for cell biologists with prior experience in light microscopy who wish to add super resolution microscopy to their research program. Participants will acquire both theoretical understanding of super resolution microscopy and practical experience using state-of-the-art super resolution microscopes.
Lecture Topics
Introduction to Super Resolution Microscopy; Advances in Super Resolution Microscopy; Theoretical Background of SIM, PALM/dSTORM, and STED Imaging; Advances in Fluorescent Protein and Organic Dye Technology; Applications of Super Resolution Microscopy using SIM and dSTORM; Challenges Associated with Obtaining Good SIM, STORM and STED Images; Potential Artifacts Common to Super Resolution Imaging.
Laboratory Topics
Working in groups of five, participants will rotate through multiple work stations.
The following work stations are located on NIH campus:
- Leica gSTED, GE- OMX SIM, Nikon N-STORM, Zeiss Elyra PALM/SIM, Zeiss LSM880-AiryScan, Shroff Lab - Instant SIM, Shroff Lab - Dual View Plane Illumination Microscope.
Another three stations are located on Janelia Farm campus including iPALM, Lattice light sheet microscope, TIRF-SIM for live cell imaging. Complimentary transportation will be provided to and from the Janelia Farm campus.
During the laboratory sessions, the following topics will be covered:
- Introduction and Feature Highlights of the Instrument
- Imaging Acquisition Procedures
- Sample Preparation Requirements and Recommendations (Dye Choices and Imaging Solutions)
- Imaging Data Analysis and Presentation
- Trouble-shoot Common Problems in SIM Imaging
- Acquisition of 2D and 3D STORM Images
Related BioTech Course
BioTech 35: Confocal & Immunofluorescence Microscopy
General Training Rate
$ 1545.00
Discounted Training Rates $ 545.00 - NIH Trainees (fellows, postdocs, postbacs - NIH ONLY) $ 1145.00 - NIH Community $1345.00 - Academia, Government, Military
Comprehensive Binder
Contains all notes, protocols, and reference material needed. No additional books are required.
Credit
Although no grades are given for BioTech Courses, each participant will receive Continuing Education Units (CEUs) based on the number of contact hours. Upon completion of this 4-day program, each participant will receive 2.8 CEUs.
Refund Policy
100% Refund or 100% Full Workshop Credit Available Written Notification: 14 or more days prior to start of program.
50% Refund or 100% Full Workshop Credit Available Written Notification: 4 to 13 days prior to start of program.
0% Refund, 100% Full Workshop Credit Available 3 days or less prior to start of program.
Notification
- All cancellations must be received in writing via email to Ms. Carline Coote at ccoote@mail.nih.gov
- Cancellations received after hours are time marked for the following day.
- In regards to a full or partial refund or a full workshop credit, you must indicate which option you are selecting at the time of your written cancellation.
- All refund payments will be processed by the start of the initial workshop.
Suggested Accommodations
The Double Tree Hotel & Executive Center