When

Saturday November 12, 2016

9:00 AM to 12:00 PM PACIFIC TIME

 
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Where

Hilton San Diego Gaslamp Quarter 
401 K Street
San Diego, CA 92101  

 
Driving Directions 

 

 

Contact

Kristin Connors 
MBF Bioscience 
802-288-9290 
kristin@mbfbioscience.com 

 

Quantitative Analysis Workshops 

Practical Stereology & Automatic Neuron Tracing

Saturday, November 12 (prior to SfN)

$50 to attend

 

Join us for a morning filled with dynamic discussions about quantitative neuron analysis.

There will be 2 sessions running concurrently from 9:00 am - 12:00 pm Pacific Time. One session is for stereology, the other is neuron reconstruction.

Both workshops will be held at the same time. Please register for one of the sessions listed below. 

Questions about which session to register for?
Contact Kristin Connors at kristin@mbfbioscience.com

Automatic neuron reconstruction and analysis

Presented by
Dr. Susan Tappan, Scientific Director, MBF Bioscience

 

 

Join me, Dr. Susan Tappan, for an exciting, interactive workshop where you will learn about the latest automatic methods for neuron reconstruction and analysis in Neurolucida 360. You will learn how to get accurate, reproducible data about the size, shape, and complexity of neurons as quickly as possible. Ample time will be given for question and answer sessions, and all attendees will get a free trial of Neurolucida 360.

You can install the free trial on your laptop PC and follow along as I give a demonstration of automatic neuron tracing with Neurolucida 360 during the workshop. If you don't have a laptop PC you can watch my screen or share with another attendee. You will have time to continue your free trial after the Society for Neuroscience meeting. This extended free trial period is exclusive to workshop attendees and provides you with opportunity to test the software with multiple data sets from your own lab.

What will we cover?  

I am prepared to cover topics that matter most to the attendees, whether that's dendritic spine analysis or neuronal tracing from Golgi-impregnated material. Neurolucida 360 can be used to analyze multiple entire neurons, or small dendritic segments.

Using Neurolucida 360 at the workshop
  
Neurolucida 360 has 3 algorithms available for neuron tracing in automated or user-guided modes.  I'll demonstrate the strengths of each algorithm, as well as how to seamless switch algorithms while tracing.  Dendritic spine modeling is also big component of Neurolucida 360. I'll demonstrate the differences between the different ways to model dendritic spines, and how you can use the metrics that are calculated from Neurolucida 360 for multiple analyses. 

Together we will use Neurolucida 360 to trace example image data and analyze the results.  Bring a powerful PC (not Mac) laptop to the workshop so  you can join in!

Bring your questions, or offer them at the time of registration!

Note: 
Neurolucida 360 can reconstruct axonal projections and other neural features in very large images.  We continue to optimize the software to work efficiently with ever-increasing image data sizes.  Neurolucida 360 is compatible with iDISCO (and the new uDISCO method) for tissue clearing, however, the SfN 2016 workshop will not focus on whole-tissue images, since those image files are too large to be easily manipulated on a laptop computer. The topics covered in the workshop, though, will be applicable to images of any size. You will receive a free trial of the software that you can install & run on a more powerful computer in your lab to work with whole-tissue images.  

Practical stereology techniques to obtain unbiased, reproducible results

Presented by
Dr. Jose Maldonado, Sr Scientific Advisor, MBF Bioscience

 

Stereology is a powerful method for quantifying cell numbers, areas, and volumes in biological structures in a reproducible and unbiased fashion. The goal of this workshop is for you to learn how to design and execute an unbiased stereology study. I will focus on the Optical Fractionator probe for quantifying cell number, and the Cavalieri probe for quantifying volume of a region. I will briefly touch on other probes such as the Area Fraction Fractionator for quantifying the volume of a sub-region (e.g. the percentage by volume of plaque in the cortex). This workshop will be a combination of short lectures, question and answer sessions, and hands-on experience using Stereo Investigator.

Attendees will get a free trial of Stereo Investigator. You can install the free trial on your laptop PC and follow along step-by-step an the optical fractionator demonstration. If you don't have a laptop PC you can watch my screen or share with another attendee.