Date:  Wednesday, March 27, 2019
Time:  11:00 AM to 2:00 PM EST

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North Carolina State - BTEC, NC

Please note that the address of the event will be included in your registration confirmation email.


Carol Stanton 
Eppendorf North America 


Complimentary Bioprocess Workshop
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Please plan to join us for lunch.

Eppendorf invites you to be our guest at a complimentary bioprocess workshop where attendees will benefit as we present and discuss information on Scalability -  Process Scale-up Made Easy Using Standard Methods and Techniques.   We will also discuss two or our recent Application Notes on rAAV Production in Suspension CAP GT® Cells and Redox Potential Monitoring for Improved Anaerobic Fermentation.

Our integrated portfolio of bioprocess systems, software and consumables will be on hand for demonstration including the DASbox®
Mini Bioreactor System, the BioFlo® 120 and BioFlo® 320 Controllers, as well as our full line of Single-use Vessels. 

The Eppendorf bioprocess solutions have been successfully used in the biopharmaceutical, chemical and nutrition industries as well as applied research for decades.  Our strong synergies in bioreactor technology and polymer manufacturing have helped Eppendorf emerge as a global player and a valuable resource to our customers.

Scalability:  Process scale-up made easy using the standard methods and techniques that you can apply to your process today.
The ultimate goal for process development is the realization of commercial production.  The scale-up of fermentation and cell culture processes are an important step for effective production of biologicals, including small proteins in the biopharmaceutical market.  Therefore, this presentation will examine the critical parameters for scaling-up cell culture and fermentation processes.  It will review the importance of the vessel and impeller geometries and how they influence the scale-up process.  In addition, it will include real life examples of common scale-up methods like constant tip speed, kLa, oxygen transfer rate (OTR), and power per volume.  These fermentation process scale-up methods will be demonstrated using E-coli in benchtop (1 L to 10 L scale) to pilot/production scale (100 L) systems whereas, the cell culture scale-up methods will be demonstrated using monoclonal antibody producing CHO cells from 0.25L to 3.0L to 40L using the BioBLU® Single-Use Bioreactor Vessels.

rAAV Production in Suspension CAP GT® Cells in Single-Use Vessels
In the pharmaceutical and biotech industry there is an increasing interest in gene therapy-based applications.  This leads to an increasing demand in scalable production systems for viral vectors. Cell culture in suspension instead of monolayer culture simplifies scale-up because it allows bioprocessing in scalable, stirred-tank bioreactors. Researchers at Cevec Pharmaceuticals adapted the small scale shake flasks rAAV production process to stirred-tank bioreactors and scaled it up in BioBLU Single-Use Vessels from a working volume of 2 L to 10 L.   Scale-up in bioreactors was based on constant power input/volume. It led to comparable cell growth and virus production at both scales and to the original shake flask process.

Redox Potential Monitoring for Improved Anaerobic Fermentation
In fermentation redox potential is an important physiochemical factor that measures the tendency of the culture medium to acquire electrons. It can directly influence bioprocess efficiency. For example, in the anaerobic fermentation of Clostridium to produce industrial solvents, the redox potential relates to the intracellular ratio of NAD(P)+ to NAD(P)H, which in turn influences the production of solvents, including butanol.  In this study, we cultivated C. beijerinckii under anaerobic conditions in BioBLU 3f Single-Use Vessels.  We monitored the redox potential of the fermentation broth online using ISM® redox sensors and observed that the redox potential varied throughout the process.  When we kept it close to 500 mV by adding sodium sulfide, bacterial growth and butanol production drastically increased compared to a process without redox potential control.  The study demonstrates the suitability of the BioFlo bioreactor systems for anaerobic fermentation and shows the advantages of redox potential monitoring during C. beijerinckii fermentation.

Lunch will be served.


Presenter:  Michelet Dorceus is the Senior Bioprocess Application Specialist at Eppendorf North America.  Michelet earned his bachelor degree in biology and chemistry from Salem State University.  Michelet has worked in process development and manufacturing in the biotechnology/biopharmaceutical industry for over 15 years.


Presenter:  Brad Stasny is the Director of Business Development for the bioprocess portfolio at Eppendorf.  Brad earned his M.S. in Immunology from the University of Connecticut and his M.B.A. in Pharmaceutical Management from Fairleigh Dickinson University.  He has over 10 years of experience in cell biology, virology, process development, scale-up and GMP manufacturing.

Register Now!

Please plan to join us for lunch.

For more information please contact Carol Stanton at (508) 740-8055 or email her at stanton.c@eppendorf.com.

Participation is free but space is limited so please be sure to click on the "Register Now!" button to register for the event and reserve your spot.  We look forward to seeing you!