This workshop will review strategies for hydrodynamic and thermodynamic analysis of sedimentation equilibrium and sedimentation velocity analytical ultracentrifugation (AUC), dynamic light scattering (DLS), small angle scattering (SAX), isothermal titration calorimetry (ITC), surface plasmon resonance (SPR), circular dichroism (CD), and fluorescence spectroscopy for the characterization of biological macromolecules in solution.

The workshop will start on Monday morning 9 am and end on Friday afternoon 5 pm. On the first day, we will provide an introduction into the experimental aspects of AUC, DLS and ITC. This part will take place in the laboratory. We will review the basic operation of the instruments, choice of buffers, and detection systems, and experimental design dependent on the system under study.  For AUC, assembling the AUC cells and operating the XLA/I will be discussed in detail.  The following days will cover many advanced topics of theory, data analysis and experimental setup. These lectures are accompanied by practical computer exercises with the software.  

The workshop is aimed at researchers with at least limited experience in AUC, DLS and/or ITC. An integral part of the workshop is the presentation by the participants of their systems of interest and informal discussion of data.

Target Audience 
The workshop is aimed at researchers with at least limited experience in AUC, DLS, ITC, CD, and/or fluorescence techniques for characterizing biomacromolecules.

Faculty
Chad Brautigam, Ph.D.
Department of Biophysics, UTSW Medical  Center, Dallas, Texas
Rodolfo Ghirlando, Ph.D.
Laboratory of Molecular Biology, NIDDK, NIH
Alexander Grishaev, Ph.D.
UMD/NIST Institute for Bioscience &  Biotechnology Research
Joseph Mindell, Ph.D.
Membrane Transport Biophysics Section, NINDS, NIH
Tomas Obsil, Ph.D.
Faculty of Science, Charles University, Prague, Czech Republic
Grzegorz Piszczek, Ph.D. 
Biophysics Core, NHLBI, NIH
Peter Schuck, Ph.D. 
Dynamics of Macromolecular Assembly Section,  LCIMB, NIBIB, NIH
Bonnie Wallace, Ph.D.
Birbeck College, University of London
Huaying Zhao, Ph.D.
Dynamics of Macromolecular Assembly Section,  LCIMB, NIBIB, NIH

Registration Fees
$1,650.00 - Industry Participants 
$1,000.00 - Academic/Government Participants  
$750.00 - Active Student Participants (Ph.D. Students, PostDocs, PostBacs, Fellows, IRTAs pending graduate or medical school)
Documentation is Required.

Registration Fee includes Morning, Afternoon Snacks and Lunch each day. 

Cancellations and Refunds
Full refunds will be issued for all cancellations, received in writing, on or before May 1, 2018. Please contact Carline Coote at (301) 496-7977 or via email, registration@faes.org, if you wish to cancel your registration.  No refunds will be issued after May 1, 2018.